El Don (ATCC® CRL-1149)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 31 years
Gender female
Clinical Data
The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No.
female
Comments
The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No. 2 in Lichtenstein, JR, et al. Science 182:298, 1973.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.
Basic Documentation
References

Lichtenstein JR, et al. Defect in conversion of procollagen to collagen in a form of Ehlers-Danlos syndrome. Science 182: 298-300, 1973. PubMed: 4742738