Detroit 529 (ATCC® CCL-66)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 2.5 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype number = 48; trisomic for X; trisomic for a group G chromosome; Down syndrome
Clinical Data
female
Caucasian
2.5 years
Virus Susceptibility Human poliovirus 1
Comments
The cells have a finite life expectancy of 30 serial passages from the tissue of origin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 13,14
D5S818: 12,13
D7S820: 10
THO1: 8,9
TPOX: 8,11
vWA: 18,19
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
Passage History
The cells have a finite life expectancy of 30 serial passages from the tissue of origin.
Year of Origin October, 1963
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation