CCD-186Sk (ATCC® CRL-1563)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 16 years
Gender male
Clinical Data
The tissue donor suffered from cystic fibrosis.
male
Genes Expressed
Homozygous for the Delta 508 mutation in the CFTR gene.
Comments
The tissue donor suffered from cystic fibrosis.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.