MUG-Chor1 (ATCC® CRL-3219)

Organism: Homo sapiens, human  /  Tissue: sacral bone  /  Disease: tumor, chordoma

Organism Homo sapiens, human
Tissue sacral bone
Product Format frozen
Morphology Mesenchymal like, with variable vacuoles
Culture Properties adherent
Biosafety Level 1
Disease tumor, chordoma
Age 57 years
Gender female
Ethnicity Caucasian
Applications
Use as a model of chordoma which is a rare slow-growing tumor

The Chordoma Foundation can offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Shipping Information frozen
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data
57 years
female
Caucasian
Genes Expressed amplification of transcription factor T (brachyury)
Comments MUG-Chor1 is a human chordoma cell line that exhibits chordoma-like characteristics and has molecular, genetic, and morphological features typical of chordoma.  Chordoma is a rare slow-growing tumor type and  MUG-Chor1 is a relatively slow-growing cell line.   MUG-Chor1 has a heterogeneous morphology consisting of physaliferous cells with mucinous intercellular substance, which represent typical chordoma features. The cells contain amplification of transcription factor T (Brachyury) that is most specific marker for chordoma, as well as loss of PTEN. This cell line was accessioned with the support of the Chordoma Foundation, a nonprofit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for chordoma.
Complete Growth Medium IMDM: RPMI-1640 (4:1) + 10% FBS
Subculturing

Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50ug/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8oC up to one week under sterile conditions.

Volumes used in this subculture protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 5.0ml Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in 10 ml fresh growth medium.
  7. Add appropriate aliquots of the cell suspension to new coated culture vessels.
  8. Incubate cultures at 37°C.
Cryopreservation Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile                 TH01: 9.3
                D5S818: 11, 12           
                D13S317: 11
                D7S820: 8, 11
                D16S539: 11, 14
                CSF1PO: 11
                Amelogenin: X
                vWA: 15
                TPOX: 8
Passage Number 20
Name of Depositor Beate Rinner, Bernadette Liegl
Year of Origin September 2009
References

Rinner B, et al. Establishment and detailed functional and molecular genetic characterisation of a novel sacral cordoma cell line, MUG-Chor1. Int J Oncol. 40(2): 443-451, 2012. PubMed: 22002331

Basic Documentation
Other Documentation
References

Rinner B, et al. Establishment and detailed functional and molecular genetic characterisation of a novel sacral cordoma cell line, MUG-Chor1. Int J Oncol. 40(2): 443-451, 2012. PubMed: 22002331