U-CH2 (ATCC® CRL-3218)

Organism: Homo sapiens, human  /  Tissue: sacral bone  /  Disease: tumor, chordoma

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Organism Homo sapiens, human
Tissue sacral bone
Product Format frozen
Morphology Mesencymal like, with variable vacuoles
Culture Properties adherent
Biosafety Level 1
Disease tumor, chordoma
Age 72 years
Gender female
Ethnicity Caucasian
Applications
Use as a model for chordoma which is a rare slow-growing tumor

The Chordoma Foundation can offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Shipping Information frozen
Storage Conditions liquid nitrogen vapor phase
Images Cell micrograph of ATCC CRL-3218 U-CH2 (human chordoma)
Derivation This cell line was established from tumor tissue obtained from a 72-year old female patient with recurrent sacral chordoma.
Genes Expressed amplification of transcription factor T (brachyury)
Comments U-CH2 is a human chordoma cell line that was established from tumor tissue obtained from a 72-year old female patient with recurrent sacral chordoma. It exhibits chordoma-like characteristics, and has molecular, genetic, and morphological features typical of chordoma.  Chordoma is a rare slow-growing tumor type, and U-CH2 is a relatively slow-growing cell line.  U-CH2 has a heterogeneous morphology consisting of physaliferous cells with mucinous intercellular substance, which represent typical chordoma features. The cells contain amplification of transcription factor T (Brachyury) that is most specific marker for chordoma. This cell line was accessioned with the support of the Chordoma Foundation, a nonprofit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for chordoma.
Complete Growth Medium IMDM:RPMI-1640 (4:1) + 10% FBS
Subculturing

Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50ug/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8°C up to one week under sterile conditions.

Volumes used in this subculture protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 5.0ml Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in 10ml fresh growth medium.
  7. Add appropriate aliquots of the cell suspension to new coated culture vessels.
  8. Incubate cultures at 37°C.
Cryopreservation Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions Temperatue: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile                 TH01: 9.3
                D5S818: 10, 11           
                D13S317: 11
                D7S820: 8, 12
                D16S539: 12
                CSF1PO: 11, 12
                Amelogenin: X
                vWA: 17
                TPOX: 8
Passage Number 20
Name of Depositor Silke Bruderlein, Peter Moller
Year of Origin September 11, 2000
References

Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487

Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487

Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078