GA-10 (Clone 4) (ATCC® CRL-2393)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Burkitt's lymphoma
Age 41 years
Gender male
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Derivation
GA-10 (Clone 4) is a subclone of the GA-10 (ATCC CRL-2392) cell line derived by culturing the parental line on a feeder layer.

GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.


 

Antigen Expression
CD77 + (90%)
Comments The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy). 

This clone of cells also appears to express high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA). 

GA-10 (Clone-4) cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete culture medium, 95%, described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Name of Depositor AP Rapoport
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.