DB (ATCC® CRL-2289™)

Organism: Homo sapiens, human  /  Tissue: Ascites  /  Cell Type: B lymphoblast

Organism Homo sapiens, human
Tissue
Ascites
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease large cell lymphoma
Age 45 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma.
Clinical Data
45 years
Caucasian
male
Antigen Expression
CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 -
Genes Expressed
CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 -
Comments
The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 to 8 X 105 cells/mL and maintain between 5 X 105 and 2 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium, 90%; DMSO, 10%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 11
THO1: 6,8
TPOX: 8
vWA: 15,16
Isotype IgG; lambda light chain
Name of Depositor M Beckwith
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723