Ramos.2G6.4C10 (ATCC® CRL-1923)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma (American)

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Burkitt's lymphoma (American)
Age 3 years
Gender male
Ethnicity Caucasian
Applications
Ramos.2G6.4C10 cells provide a sensitive and specific assay system for IL-4 that is insensitive to the presence of other lymphokines and cytokines such as interleukins (IL-2, IL-2 and IL-6), colony stimulating factors (G-CSF, M-CSF and GM-CSF) and interferons alpha and gamma (IFN alpha, IFN gamma). Tumor necrosis factors alpha and beta (TNF alpha, TNF beta) cause only minor interference.
The assay is sensitive to IL-4 concentrations in the range 50 to 800 pg/mL.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from the Ramos (RA-1) cell line (see ATCC CRL-1596) by repetitive cloning with selection for low constitutive expression of CD23 and maximum stimulation of CD23 expression by IL-4.
Clinical Data
3 years
Caucasian
male
Antigen Expression
CD23 +
Receptor Expression
interleukin 4 (IL-4); low affinity IgE (CD23)
Genes Expressed
immunoglobulin (surface and secreted)
Comments

Both the parental line (ATCC CRL-1596) and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 x 10 5 viable cells/mL and maintain between 1 and 2 x 10 6 cells/mL.
Medium Renewal: Add fresh medium every 4 to 6 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Isotype IgM
Name of Depositor JP Siegel
References

Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726