ST486 (ATCC® CRL-1647)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: ascites  /  Disease: Burkitt's lymphoma

Organism Homo sapiens, human
Tissue
ascites
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Burkitt's lymphoma
Gender female
Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
Antigen Expression
HLA A2, A10 (Aw25), B5 (Bw52), B18
Receptor Expression
complement
Genes Expressed
IgM; kappa light chain, immunoglobulin (surface and secreted)
Cellular Products
IgM; kappa light chain
immunoglobulin (surface and secreted)
Comments
The cells are EBNA negative and are negative for Fc receptors.
Approximately 2% of the cells express commplement receptors.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 9,12
D5S818: 11,12
D7S820: 7,12
THO1: 7,9
TPOX: 8,11
vWA: 17,18
Population Doubling Time 19 to 20 hrs
Name of Depositor IT Magrath
References

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722

Basic Documentation
References

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722