SCC-25 (ATCC® CRL-1628)

Organism: Homo sapiens, human  /  Tissue: tongue  /  Disease: squamous cell carcinoma

Organism Homo sapiens, human
Tissue tongue
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Age 70 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
70 years
Genes Expressed
epidermal keratins; low levels of involucrin
Cellular Products
epidermal keratins; low levels of involucrin
Tumorigenic Yes
Effects
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
SCC-25 cells are capable of only limited proliferation in semi-solid medium.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate and supplemented with 400 ng/ml hydrocortisone and 10% fetal bovine serum
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: Cells are disaggregated to single cells when the culture is preconfluent. They do not require a feeder layer.
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 11,12
D5S818: 12
D7S820: 12
THO1: 8
TPOX: 8,12
vWA: 17,19
Name of Depositor JG Rheinwald
References

Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336

Basic Documentation
Other Documentation
References

Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336