TCCSUP (ATCC® HTB-5)

Organism: Homo sapiens, human  /  Tissue: urinary bladder  /  Disease: grade IV transitional cell carcinoma

Organism Homo sapiens, human
Tissue urinary bladder
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade IV transitional cell carcinoma
Age 67 years
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype (P12 and 35) hypotetraploid with marker chromosomes
Derivation
The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder.
Clinical Data
female

Antigen Expression
HLA 2, 3, 7, 12
Comments

The patient had a 4 month history of hematuria prior to removal of the tumor.

Metastases to the bone marrow were discovered later.

Studies on ultrastructure indicated presence of microvilli and lipid bodies but no desmosomes were observed.
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids (ATCC 30-2003) and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended 

Medium Renewal: 2 to 3 times per week Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9,11
D5S818: 12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 14,16
Isoenzymes
AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 1
Name of Depositor C O'Toole
References

O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756

Basic Documentation
Other Documentation
References

O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756