NCI-H596 [H596] (ATCC® HTB-178)

Organism: Homo sapiens, human  /  Tissue: lung  /  Disease: adenosquamous carcinoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue
lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenosquamous carcinoma
Age 73 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 71; range = 65 to 75.
This is a near triploid human cell line. The modal chromosome number was 71 although cells with 70 chromosomes occurred frequently. The frequency of higher ploidies was 2.9%.; Over 11 marker chromosomes were common to all cells, and at least 6 others were found in some cells. Among the common markers were: del(1)(p22), i(3q), i(6p), i(7p), t(7p17q), 14q+ and five others.; Of these, del(1) and i(7p) were generally double [occasionally triple for del(1)]. Constantly, there were 2 to 3 minute chromosomes that were about 1/2 the size of a G group chromosome.; Structurally normal N17 had only a single copy per cell, whereas N15 had four copies and N20 and N21 had four or more copies. The X chromosome was paired, but the Y was not found by fluorescence microscopy.
Images
Derivation
The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.
The specimen was obtained prior to therapy.
Clinical Data
73 years
Caucasian
male
The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein.
The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12,13
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 11
THO1: 7,9.3
TPOX: 8
vWA: 16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1983
References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140