Panc 10.05 (ATCC® CRL-2547™) Organism: Homo sapiens, human / Tissue: pancreas / Cell Type: epithelial General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue pancreas Cell Type epithelial Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Disease adenocarcinoma Age adult Gender Male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Images Derivation Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558). Clinical Data adult male Markers MHC class I +; MHC class II - Genes Expressed cytokeratins 7 and 18, K-ras + Tumorigenic Yes Comments Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The cells have a reported plating efficiency of 40%. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 10 Units/ml human recombinant insulin fetal bovine serum to a final concentration of 15% Subculturing Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase Culture Conditions Temperature: 37°C STR Profile D5S818: 13 D13S317: 12 D7S820: 8,9 D16S539: 9,12 vWA: 16 THO1: 6,9.3 Amelogenin: X TPOX: 11 CSF1PO: 12 Population Doubling Time 19.2 hrs Name of Depositor EM Jaffee Year of Origin 1992 References Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS Other Documentation Cancer cell line mutation data Cell Micrograph References Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602