SK-N-AS (ATCC® CRL-2137™)

Organism: Homo sapiens, human  /  Tissue: brain; bone marrow  /  Cell Type: neuroblast

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue
brain; bone marrow
Cell Type neuroblast
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease neuroblastoma
Age 6 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
6 years
Caucasian
female
Receptor Expression
insulin-like growth factor I (IGF-I), expressed
Tumorigenic Yes
Comments
SK-N-AS cells synthesize Insulin-like growth factor II (IFG-II) and IGF-II RNA and possess type I IGF receptors [Pubmed ID: 2547840]. Retinoic acid partially inhibits proliferation, and cells fail to differentiate [Pubmed ID: 1751405]. The cells exhibit low or no MDR1 expression.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • O.1 mM Non-Essential Amino Acids (NEAA)
  • fetal bovine serum to a final concentration of 10%
Subculturing
Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 MM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
Population Doubling Time 39 hrs
Name of Depositor C Helson
Year of Origin 1981
References

Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405

El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840

bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405

El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840

bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma