HT-1197 (ATCC® CRL-1473)

Organism: Homo sapiens, human  /  Tissue: urinary bladder  /  Disease: carcinoma

Organism Homo sapiens, human
Tissue urinary bladder
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease carcinoma
Age 44 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
44 years
Caucasian
male
Genes Expressed
fibrinolytic activity
Cellular Products
fibrinolytic activity
Tumorigenic Yes
Effects
Yes, in mice and hamsters
Comments
The cells will also grow in soft agar.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium. 
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.
Subcultivation Ratio: Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Twice per week
Cryopreservation
Storage Temperature: Liquid nitrogen vapor phase
Isoenzymes
G6PD, B
Name of Depositor S Rasheed
References

Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628

Basic Documentation
Other Documentation
References

Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628