SW948 [SW-948] ATCC ® CCL-237™ Homo sapiens colon Dukes' typ

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	colon
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	Dukes' type C, grade III, colorectal adenocarcinoma
Age	81 years
Gender	female
Ethnicity	Caucasian

Clinical Data	female 81 years Caucasian
Antigen Expression	blood type O; Rh+
Oncogene	myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Genes Expressed	carcinoembryonic antigen (CEA) 7 ng/10 exp6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin,myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src -,blood type O; Rh+,The cells are positive for keratin by immunoperoxidase staining.,The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes.
Tumorigenic	Yes
Comments	The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes. N-myc and sis oncogene expression were not detected.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.
Culture Conditions	Atmosphere: air, 100%

STR Profile	Amelogenin: X CSF1PO: 12 D13S317: 10,11 D16S539: 11,12 D5S818: 11 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 16,18
Isoenzymes	ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2

Name of Depositor	A Leibovitz
References	Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Name of Depositor

A Leibovitz

References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cancer cell line mutation data
References	Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

SW948 [SW-948] (ATCC® CCL-237™)