U-118 MG (ATCC® HTB-15)

Organism: Homo sapiens, human  /  Cell Type: Glioblastoma  /  Tissue: brain  /  Disease: classified as grade IV as of 2007, glioblastoma; astrocytoma

Organism Homo sapiens, human
Tissue
brain
Cell Type Glioblastoma
Product Format frozen
Morphology mixed
Culture Properties adherent
Biosafety Level 1
Disease classified as grade IV as of 2007, glioblastoma; astrocytoma
Age 50 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-16) by J. Ponten and associates from 1966 to 1969.
Clinical Data
50 years
Caucasian
male
Antigen Expression
Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47
Tumorigenic Yes
Effects
Yes, in nude mice inoculated subcutaneously with 10(7) cells
Comments
NOTE: The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
Mycoplasma contamination was eliminated in 1987 by treatment with BM-Cycline over a six week culture period.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapot temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
Isoenzymes
AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 2
Name of Depositor J Ponten
Year of Origin 1966
References

Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221

Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

Tumors developed within 21 days at 100% frequency (5/5).

Basic Documentation
Other Documentation
References

Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221

Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

Tumors developed within 21 days at 100% frequency (5/5).