MDA-MB-415 (ATCC® HTB-128)

Organism: Homo sapiens, human  /  Tissue: mammary gland/breast; derived from metastatic site: pleural effusion  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue mammary gland/breast; derived from metastatic site: pleural effusion
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 38 years
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number =73; range = 65 to 76. The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations.
Clinical Data
female
Caucasian
Antigen Expression
Blood Type O
Genes Expressed
amelogenin (X chromosome)(AMELEX)
Tumorigenic No
Effects
Yes, in semisolid medium
No, in immunosuppressed mice
Comments

The cells express the WNT7B oncogene [PubMed: 8168088].

The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells.

The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. 

It is not amenable to trypsinization.

Complete Growth Medium Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,13
D16S539: 13
D5S818: 11,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 2
G6PD, A
GLO-I, 1-2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor R Cailleau
Year of Origin 1975
References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088

Basic Documentation
Restrictions

The ATCC Material is available with the following restrictions: The ATCC Material may not be used for commercial purposes. Purchaser will not make commercial use of the ATCC Material without obtaining a license from the University of Texas MD Anderson Cancer Center (“UTMDACC”). With respect to research use, prior to purchase, for-profit or commercial entities must first obtain a research use license from UTMDACC. For instructions on how to proceed, please contact Dustin J. Romine, M.A. at the UTMDACC Office of Technology Commercialization via email at dromine@mdanderson.org.

References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088