TUR (ATCC® CRL-2367)

Organism: Homo sapiens, human  /  Cell Type: histocyte  /  Disease: histiocytic lymphoma

Organism Homo sapiens, human
Cell Type histocyte
Product Format frozen
Morphology monocyte
Culture Properties suspension
Biosafety Level 1
Disease histiocytic lymphoma
Age 37 years
Gender male
Ethnicity Caucasian
Derivation
TUR (TPA-U937-Resistant) is a stably transfected cell line generated by Ralf Hass and Masanori Hirano in 1992 from U-937 (ATCC CRL-1593.2), a monoblastoid cell line.
U-937 cells were transfected by electroporation with the pMG2neoPA plasmid containing the neomycin resistant gene and then selected in medium containing G418 and 12-O-tetradecanoylphorbol-13-acetate (TPA).
Clinical Data
37 years
Caucasian, White
male
Antigen Expression
HLA DR
Comments
TUR cells have remained resistant to both G418 and TPA after culture in the absence of these agents for over 100 passages.

The cell line retains monocytic properties but does not differentiate along the macrophage pathway by phorbal ester treatment.

While treatment of human U-937 myeloid leukemia cells with TPA is associated with growth arrest and induction of monocytic differentiation, the TUR cell line is unresponsive to the growth-inhibitory effects of this agent.

The TUR variant is defective in TPA-induced signaling events upstream to activation of Raf-1 kinase.

Expression of major histocompatibility complex (MHC) Class II antigens on U937 and TPA-treated U937 cells is barely detectable.

However, there was a significantly constitutive expression of MHC class II, particularly human lymphocyte antigen (HLA-DR) on the surface of TUR and TPA-treated TUR cells.

TUR cells continue to proliferate in the presence of TPA although increasing concentrations of TPA continuously reduces the proliferative capacity of the cells.

Unlike U-937 cells, exposure to TPA did not induce detectable levels of internucleosomal DNA fragmentation or the generation of apoptotic bodies.



Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 14,16
Name of Depositor R Hass, I Prudovsky
Passage History
TUR cells have remained resistant to both G418 and TPA after culture in the absence of these agents for over 100 passages.
References

Hass R, et al. Resistance to phorbol ester-induced differentiation of a U-937 myeloid leukemia cell variant with a signaling defect upstream to Raf-1 kinase. Cell Growth Differ. 4: 657-663, 1993. PubMed: 8398907

Hass R, et al. Characterization of human TUR leukemia cells: continued cell cycle progression in the presence of phorbol ester is associated with resistance to apoptosis. Eur. J. Cell Biol. 65: 408-416, 1994. PubMed: 7720732

Hass R, Lopez-Guerrero JA. Aggressive tumor growth of human TUR leukemia cells is associated with high levels of c-myc expression and down-regulation of p20-max. Int. J. Cancer 72: 1113-1116, 1997. PubMed: 9378547

Meinhardt G, et al. Signaling defect in the activation of caspase-3 and PKCdelta in human TUR leukemia cells is associated with resistance to apoptosis. Exp. Cell Res. 247: 534-542, 1999. PubMed: 10066381

Basic Documentation
References

Hass R, et al. Resistance to phorbol ester-induced differentiation of a U-937 myeloid leukemia cell variant with a signaling defect upstream to Raf-1 kinase. Cell Growth Differ. 4: 657-663, 1993. PubMed: 8398907

Hass R, et al. Characterization of human TUR leukemia cells: continued cell cycle progression in the presence of phorbol ester is associated with resistance to apoptosis. Eur. J. Cell Biol. 65: 408-416, 1994. PubMed: 7720732

Hass R, Lopez-Guerrero JA. Aggressive tumor growth of human TUR leukemia cells is associated with high levels of c-myc expression and down-regulation of p20-max. Int. J. Cancer 72: 1113-1116, 1997. PubMed: 9378547

Meinhardt G, et al. Signaling defect in the activation of caspase-3 and PKCdelta in human TUR leukemia cells is associated with resistance to apoptosis. Exp. Cell Res. 247: 534-542, 1999. PubMed: 10066381