HT (ATCC® CRL-2260™) Organism: Homo sapiens, human / Tissue: ascites / Cell Type: B lymphoblast General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue ascites Cell Type B lymphoblast Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease diffuse mixed lymphoma Age 70 years Gender male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Derivation HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. Clinical Data 70 yearsCaucasianmale Markers CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - Genes Expressed immunoglobulin; kappa light chain,CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - Comments HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface. Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition. HT cells have been reported to be Epstein-Barr virus genome negative. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 105 viable cells/mL and maintain between 1 X 105 and 1 X 106. Medium Renewal: Every 2 to 3 days Cryopreservation Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase Culture Conditions Temperature: 37°C STR Profile Amelogenin: X,YCSF1PO: 10D13S317: 13,14D16S539: 11,13D5S818: 11,13D7S820: 8,10THO1: 6,7TPOX: 11vWA: 17,18 Isotype kappa Name of Depositor M Beckwith Year of Origin 1983 References Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723