NCI-H508 [H508] (ATCC® CCL-253)

Organism: Homo sapiens, human  /  Tissue: cecum  /  Disease: colorectal adenocarcinoma

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Organism Homo sapiens, human
Tissue cecum
Product Format frozen
Morphology epithelial
Culture Properties floating aggregates of round cells with some attached cells
Biosafety Level 1
Disease colorectal adenocarcinoma
Age 55 years
Gender male
Ethnicity Caucasian
Karyotype modal number = 102; range = 71 to 131; most cells have DM's
Derivation
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
Clinical Data
55 years
Caucasian
male
Antigen Expression
Blood type A; Rh+; CA19-9 antigen
Genes Expressed
carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days
Cellular Products
carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days
Tumorigenic Yes
Effects

Yes, in nude mice

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells

 

Comments

The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove culture medium. Keep floating cells. 
  2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C.to facilitate dispersal.
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  4. Add appropriate aliquots of the cell suspension and the harvested floating cells to new culture vessels. 
  5. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 3 times per week


Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 12
D7S820: 8,12
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

The line is available with the following restrictions:

  1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
  2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249