SW948 [SW-948] (ATCC® CCL-237)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: Dukes' type C, grade III, colorectal adenocarcinoma

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Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type C, grade III, colorectal adenocarcinoma
Age 81 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
81 years
Caucasian
Antigen Expression blood type O; Rh+
Oncogene myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Genes Expressed
carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 mL cell sonicate; keratin
Cellular Products
carcinoembryonic antigen (CEA) 7 ng/10 exp6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin
Tumorigenic Yes
Effects

Yes, in nude mice

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. 

Comments
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes.

N-myc and sis oncogene expression were not detected.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended

Medium Renewal: 1 to 2 times per week

Culture Conditions
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,18
Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1-2
PGM3, 1-2
Name of Depositor A Leibovitz
References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066