COLO 829 (ATCC® CRL-1974)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: skin  /  Disease: Melanoma

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Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Melanoma
Age 45 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The COLO 829 cell line was established by G.E. Moore from a biopsy specimen taken from a patient prior to chemotherapy for metastatic melanoma.

Clinical Data
45 years
Caucasian
male
Comments
A cell line was also produced from peripheral blood B cells, and is available as COLO 829BL (ATCC CRL-1980)
Early passages of COLO 829 still contain a small percentage of melanin producing cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of Trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19
Name of Depositor GE Moore
Passage History
Early passages of COLO 829 still contain a small percentage of melanin producing cells.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation