SW 626 [SW-626, SW626] (ATCC® HTB-78)

Organism: Homo sapiens, human  /  Tissue: ovary  /  Disease: grade III, adenocarcinoma

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Organism Homo sapiens, human
Tissue ovary
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade III, adenocarcinoma
Age 46 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Hypertetraploid; modal number = 104. The rate of higher ploidies was 23%. The markers der(2)t(2;5)(q35;q31); del(8)(q13q22); del(12)(q13); t(q9q13) and two others were common to most cells. Generally there were two copies of der(2) and three copies of del(8) per cell. The t(3;11)(p21;q25) and i(15q) markers were seen in some cells. Many cells had 8 copies of N3, N7, N9, N19 and N20, but only two copies of N2. Normal 8 was absent. There were four copies of X, and Y was not found.
Derivation
The SW 626 cell line was initiated by A. Leibovitz in January 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen from a cystadenocarcinoma of the ovary in a 46 year old female Caucasian.
Although originally thought to be of ovarian origin , a recent report has suggested that the SW 686 cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon.
Clinical Data
46 years
Caucasian
female
Tumorigenic Yes
Effects
Yes, in nude mice produces well differentiated papillary adenocarcinomas consistent with ovarian primary
Comments
The histopathology of the specimen was determined to be grade III adenocarcinoma.
A frozen ampule at passage 10 was received at the ATCC in January, 1982.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor A Leibovitz
Passage History
A frozen ampule at passage 10 was received at the ATCC in January, 1982.
Year of Origin January, 1974
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Strobel T, et al. BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc. Natl. Acad. Sci. USA 93: 14094-14099, 1996. PubMed: 8943066

Furlong MT, et al. Evidence for the colonic origin of ovarian cancer cell line SW626. J. Natl. Cancer Inst. 91: 1327-1328, 1999. PubMed: 10433623

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Strobel T, et al. BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc. Natl. Acad. Sci. USA 93: 14094-14099, 1996. PubMed: 8943066

Furlong MT, et al. Evidence for the colonic origin of ovarian cancer cell line SW626. J. Natl. Cancer Inst. 91: 1327-1328, 1999. PubMed: 10433623