Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 51695)

Strain Designations: BDMS 18 AN - PRODUCT SHEET TEST  /  Type Strain: no  /  Biosafety Level: 2

Permits And Restrictions

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Strain Designations BDMS 18 AN - PRODUCT SHEET TEST
Application
Quality control strain for Clostridium difficile agar
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Enteric Research
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Genotype Toxinotype: 0
Name of Depositor Becton Dickinson Microbiology Systems
Special Collection NCRR Contract
Chain of Custody
ATCC <-- Becton Dickinson Microbiology Systems <-- J Aswell <-- Johns Hopkins Univ. Hosp. Lab
References

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation