Geobacillus stearothermophilus (Donk) Nazina et al. (ATCC® 12980)

Strain Designations: NCA 26  /  Type Strain: yes  /  Biosafety Level: 1

Deposited As Bacillus stearothermophilus Donk
Strain Designations NCA 26
Produces restriction endonuclease BstPI
Steam sterilization control
Sterility assurance
Pharmaceutical and Personal Care
Type Strain yes
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain yes
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 55°C
Atmosphere: Aerobic
Name of Depositor Nat. Canners Assn.

Can. J. Microbiol. 6: 557, 1960.

J. Gen. Microbiol. 34: 270, 1964.

Pugatsch T, Weber H. A thermostable, sequence-specific restriction endonuclease from Bacillus stearothermophilus: BstPI. Nucleic Acids Res. 7: 1429-1444, 1979. PubMed: 503858

U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.

Nazina TN, et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and .... Int. J. Syst. Evol. Microbiol. 51: 433-446, 2001. PubMed: 11321089

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.

Cross References

Nucleotide (GenBank) : U34874 Bacillus stearothermophilus levansucrase (surB) gene, complete cds.

Nucleotide (GenBank) : X93600 B.stearothermophilus aspC gene.

Nucleotide (GenBank) : M57457 B.stearothermophilus alpha amylase gene, complete cds.

Nucleotide (GenBank) : U34875 Bacillus stearothermophilus levanase (surC) gene, complete cds.

Nucleotide (GenBank) : M63307 Bacillus stearothermophilus histidine utilization operon (hut) gene sequence.

Nucleotide (GenBank) : M26646 B.stearothermophilus manganese superoxide dismutase (MnSOD) gene, complete cds.

Nucleotide (GenBank) : AF055578 Bacillus stearothermophilus surface layer protein precursor (sbsC) gene, complete cds.

Nucleotide (GenBank) : AF139535 Bacillus stearothermophilus trp RNA-binding attenuation protein (mtrB) gene, complete cds.

Nucleotide (GenBank) : AF162268 Bacillus stearothermophilus insertion sequence ISBst12 putative transposase gene, complete cds.

Nucleotide (GenBank) : AF139534 Bacillus stearothermophilus chorismate mutase (aroH), anthranilate synthase subunit (trpE), and anthranilate phophoribosyl transferase (trpD) genes, complete cds.