Chlamydia trachomatis (ATCC® VR-901B)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

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Classification Chlamydiaceae, Chlamydia
Deposited As Lymphogranuloma venereum
Agent Chlamydia trachomatis
Strain Lymphogranuloma venereum (LGV I) strain 440
Applications
Sexually Transmitted Disease Research
Biosafety Level 3
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

This preparation has tested positive for Mycoplasma contamination.

Centrifuge at 2000 X g for 1 hour; adsorb 1 hour at 35-36°C. Feed with 1.0 mL EMEM (HEPES buffered) 5mg glucose/mL, 0.3 mg glutamine/mL, 1-2 µg/mL cycloheximide. Dilute high titer stocks to avoid cell toxicity.

Tissue culture adapted at ATCC.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent.
Incubation: 2-3 days, a 5% CO2 in air atmosphere is recommended
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Name of Depositor J Schachter
Source Lymph node from human with LGV
References

Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618

Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529

Cross References

Nucleotide (GenBank) : AJ012175 Chlamydia trachomatis partial rnpB gene for RNase P RNA subunit.

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