Chlamydia trachomatis Serovar D (ATCC® VR-885)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Chlamydiaceae, Chlamydia
Agent Chlamydia trachomatis Serovar D
Strain Trachoma type D strain UW-3/Cx
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Rapid loss in titer when stored above -70°C.

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL Cycloheximide.

Effect on Host
CPE, intracellular inclusion bodies
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, 5% CO2 in air atmosphere is recommended
Effect on Host
CPE, intracellular inclusion bodies
Name of Depositor SP Wang
Source Human cervix, asymptomatic
References

Alexander ER. . Am. J. Opthalmol. 63: 1469, 1967.

Holt S. Am. J. Opthalmol. 63: 1057, 1967.

Wang SP. Am. J. Opthalmol. 70: 367, 1970.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation