Chlamydia trachomatis Serovar D (ATCC® VR-885™)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydia
Agent Chlamydia trachomatis Serovar D
Strain Trachoma type D strain UW-3/Cx
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Rapid loss in titer when stored above -70°C.

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL Cycloheximide.

Effect on Host
CPE, intracellular inclusion bodies
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, 5% CO2 in air atmosphere is recommended
Effect on Host
CPE, intracellular inclusion bodies
Name of Depositor SP Wang
Source Human cervix, asymptomatic
References

Alexander ER. . Am. J. Opthalmol. 63: 1469, 1967.

Holt S. Am. J. Opthalmol. 63: 1057, 1967.

Wang SP. Am. J. Opthalmol. 70: 367, 1970.