Chlamydia trachomatis (Busacca) Rake (ATCC® VR-878™)

Classification: Chlamydiaceae  /  Product Format: frozen 1 mL per vial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Chlamydiaceae
Agent Chlamydia trachomatis (Busacca) Rake
Strain UW-57/Cx
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for one hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 36°C for 72 hours.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Name of Depositor SP Wang
Source Human cervix (epithelial tissue), cervicitis, Seattle, WA, 1971
References

Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS