Chlamydophila pecorum (Fukushi and Hirai) Everett et al. ATCC ® VR

Permits	These permits may be required for shipping this product: This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Chlamydiaceae
Agent	Chlamydophila pecorum (Fukushi and Hirai) Everett et al.
Strain	Sporadic Bovine Encephalomyelitis [strain E58 (McNutt)]
Biosafety Level	2
Isolation	clinical specimen - animal United States
Product Format	frozen
Storage Conditions	-70°C or colder

Comments	Growth rate markedly stimulated by increases in incubation temperature. Optimum temperature for growth in CE is 39°C. Previously classified as C. psittaci; see Fukushi & Hirai (IJSB. 42: 306, 1992) for pecorum classification.
Effect on Host	Death in chicken embryo with hyperemia and hemorrhage in yolk sac and embryo In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo In cell culture produces inclusions which can be visualized by fluorescent antibody staining

Recommended Host	McCoy (ATCC CRL-1696)
Growth Conditions	Incubation: chicken embryo: 7-10 days at 39°C with humidity; tissue culture: 48 to 72 hours at 37°C, 5% CO₂ in air atmosphere is recommended

Effect on Host	Death in chicken embryo with hyperemia and hemorrhage in yolk sac and embryo In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo In cell culture produces inclusions which can be visualized by fluorescent antibody staining

Name of Depositor	LA Page
Source	clinical specimen - animal United States
References	McNutt SH. . Cornell Vet. 30: 437, 1940. Fukushi H, Hirai K. Proposal of Chlamydia pecorum sp. nov. for Chlamydia strains derived from ruminants. Int. J. Syst. Bacteriol. 42: 306-308, 1992. PubMed: 1581191 type strain y. s. inoculation calf brain, herd in Iowa
Cross References	Nucleotide (GenBank) AJ012173 Chlamydophila pecorum partial rnpB gene for RNase P RNA subunit. Nucleotide (GenBank) U68433 Chlamydia pecorum E58 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence.

Permits

These permits may be required for shipping this product:

This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation

Chlamydophila pecorum (Fukushi and Hirai) Everett et al. (ATCC® VR-628™)