Chlamydophila pecorum (Fukushi and Hirai) Everett et al. (ATCC® VR-628)

Classification: Chlamydiaceae  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae
Agent Chlamydophila pecorum (Fukushi and Hirai) Everett et al.
Strain Sporadic Bovine Encephalomyelitis [strain E58 (McNutt)]
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Growth rate markedly stimulated by increases in incubation temperature. Optimum temperature for growth in CE is 39°C. Previously classified as C. psittaci; see Fukushi & Hirai (IJSB. 42: 306, 1992) for pecorum classification.

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

 

Effect on Host
In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo
Recommended Host
McCoy (ATCC CRL-1696)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent as the cylcoheximide will prevent further growth.
Incubation: 48 to 72 hours, a 5%CO2 in air atmosphere is recommended.
Effect on Host
In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo
Name of Depositor LA Page
Source Calf brain from herd in Iowa, USA
References

Fukushi H, Hirai K. Proposal of Chlamydia pecorum sp. nov. for Chlamydia strains derived from ruminants. Int. J. Syst. Bacteriol. 42: 306-308, 1992. PubMed: 1581191

McNutt SH. . Cornell Vet. 30: 437, 1940.

Cross References

Nucleotide (GenBank) : AJ012173 Chlamydophila pecorum partial rnpB gene for RNase P RNA subunit.

Nucleotide (GenBank) : U68433 Chlamydia pecorum E58 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence.