Chlamydia trachomatis (Busacca) Rake (ATCC® VR-573™)

Classification: Chlamydiaceae  /  Product Format: frozen

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Classification Chlamydiaceae
Agent Chlamydia trachomatis (Busacca) Rake
Strain Trachoma type B strain HAR-36
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Isolation
clinical specimen - human
Saudi Arabia
Product Format frozen
Storage Conditions -70°C or colder
Comments
Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Egg preparations may require adaptation to grow in tissue culture cells.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Toxic for white mice in 1 to 24 hours when given i. v.
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions
Temperature: 37.0°C
Incubation: incubate 48 to 72 hours at 37°C in tissue culture
Protocol: Add glassbeads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1 to 2 mcg/ml cycloheximide. Incubate at 37C for 48 to 72 hours.
Subcultivation
Protocol: Add glassbeads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1 to 2 mcg/ml cycloheximide. Incubate at 37C for 48 to 72 hours.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Toxic for white mice in 1 to 24 hours when given i. v.
Name of Depositor RL Nichols
Special Collection ATCC
Source
clinical specimen - human
Saudi Arabia
References

Bell SD, Fraser CE. Experimental trachoma in owl monkeys. Am. J. Trop. Med. Hyg. 18: 568-572, 1969. PubMed: 4978598

human conjunctiva from a 12-month-old male patient with trachoma (TR II follicles and papillae)

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS