Chlamydia trachomatis Serovar Ba (ATCC® VR-347)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydia
Deposited As Trachoma serotype ba
Agent Chlamydia trachomatis Serovar Ba
Strain Apache-2
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images Chlamydia trachomatis ATCC VR-347 Images
Comments FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Please see ATCC Virology Guide for more detailed information on propagation.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host
McCoy cells (ATCC CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.  Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).  Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.  End adsorption by adding agent growth medium.
Incubation: Incubate infected culture for 72 hours at 36°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled rate freeze.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor E Jawetz
Special Collection ATCC
Source Eye of an Apache Indian child from San Carlos, Arizona
References

Hanna L, et al. Trachoma viruses isolated in United States. I. Growth in embryonated eggs. Proc. Soc. Exp. Biol. Med. 104: 142-145, 1960. PubMed: 14399529