Chlamydia trachomatis (Busacca) Rake (ATCC® VR-346™)

Classification: Chlamydiaceae  /  Product Format: frozen 1 mL per vial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Chlamydiaceae
Agent Chlamydia trachomatis (Busacca) Rake
Strain Trachoma type F strain IC-Cal-3
Applications
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Rapid loss in titer when stored above -70°C. Aspirate fluid several times with needle and syringe or vortex for 45 seconds with 1mm glass beads. Infect monolayer with 0.1ml of 1:10 material. Centrifuge at 2000g for 1 hour; adsorb for 1 hour.

REFERENCE STRAIN: Trachoma Group, International Congress of Microbiology, 1962.

Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Add glassbeads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 mg/mL cycloheximide. Incubate at 37°C for 48 hours, a 5% CO2 in air atmosphere is recommended.  
Incubation: 48 hours
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies
Name of Depositor L Hanna, E Jawetz, Centers for Disease Control and Prevention
Source Eye of nine-day-old baby, California, 1960
References

Hanna L. . Am. J. Opthalmol. 53: 774, 1962.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS