Rickettsia raoultii (ATCC® VR-1596)

Classification: Rickettsiaceae, Rickettsieae  /  Product Format: frozen

Classification Rickettsiaceae, Rickettsieae
Agent Rickettsia raoultii
Strain DnS14
Applications
Vector borne research
Positive for Mycoplasma Yes
Biosafety Level 3
Isolation
Isolated from Dermacentor nutalli in Siberia, in 1994
Product Format frozen
Storage Conditions  Store at -60°C or colder
Comments
This item is a representative of a newly identified rickettsial species (Rickettsia raoultii), a gram-negative, obligately intracellular and non-motile bacterium.
It is genetically identical to the type strain for this species (Khabarovsk, isolated in 2005) at all loci sequenced. The preparation available through ATCC tested positive for mycoplasma contamination (M.orale) by PCR.
Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling.
Bacteria visible in cells following staining with Gimenez stain
Effect on Host
CPE in the form of cell rounding and sloughing of infected cells.
Growth Medium Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Vero cells (ATCC CRL-1587)
Growth Conditions
Recommendation for Infection: 5 days at 32°C and 5% CO2 in EMEM (ATCC 30-2003; with 2 mM L-glutamine) supplememted with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C
Effect on Host
CPE in the form of cell rounding and sloughing of infected cells.
Name of Depositor Dr. P. -E. Fournier
Special Collection ATCC
Year of Origin 1994
References

Mediannikov, O., et. al. 2008. Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia. Int. J Syst. Evol. Microbiol. Jul;58 (Pt 7):1635-39. PubMed: 18599708

Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804

Rydkina, E. et. al., New Rickettsiae in ticks collected in territories of the former soviet union. Emerg. Infect. Dis. 1999. Nov-Dec;5(6):811-4. PubMed: 10603217