Chlamydophila pecorum (Fukushi and Hirai) Everett et al. (ATCC^® VR-1575^™)

Classification: Chlamydiaceae / Product Format: frozen

Chlamydophila pecorum (Fukushi and Hirai) Everett et al. ATCC^® VR-1575^™

frozen

For-Profit: $516.00 Non-Profit: $430.00

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Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Chlamydiaceae
Agent	Chlamydophila pecorum (Fukushi and Hirai) Everett et al.
Strain	Ovine Polyarthritis strain IPA (Iowa polyarthritis)
Biosafety Level	2
Product Format	frozen
Storage Conditions	-70°C or colder

Effect on Host	in vitro effects: CPE, subtle - monolayer dirty looking with cell degeneration Subtle CPE; monolayer dirty looking with cell degeneration.

Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 0.002 mg/ml cyclohexamide, 10 mM HEPES, 90%; fetal bovine serum (chlamydial antibody-free, e.g., Biowhittaker 14-902E), 10%
Recommended Host	McCoy ATCC CRL-1696 alternate: chicken embryo alternate: guinea pig alternate: turkey Recommended Host: McCoy (ATCC^® CRL-1696^TM)
Growth Conditions	Temperature: 37.0°C Duration: 48 to 72 hours. For best results cells should be 24 to 48 hours old and 90-100% confluent. Atmosphere: 5% CO2 in air recommended.

Effect on Host	in vitro effects: CPE, subtle - monolayer dirty looking with cell degeneration Subtle CPE; monolayer dirty looking with cell degeneration.

Name of Depositor	LA Page, ATCC
Source	derived from existing strain (This is a tissue culture adapted version of ATCC VR-629. ATCC VR-629 was isolated from sheep's joint fluid in Iowa.)
References	Page LA, Cutlip RC. Chlamydial polyarthritis in Iowa lambs. Iowa Vet. 39: 10-18, 1968.

Permits

These permits may be required for shipping this product:

Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation