Chlamydia trachomatis (Busacca) Rake (ATCC® VR-1477™)

Classification: Chlamydiaceae  /  Product Format: frozen

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Chlamydiaceae
Agent Chlamydia trachomatis (Busacca) Rake
Strain TW-3 [Trachoma type C strain TW-3]
Applications
Sexually Transmitted Disease Research
Positive for Mycoplasma Yes
Biosafety Level 2
Isolation
specimen from human conjunctiva, Taiwan
Product Format frozen
Storage Conditions -70°C or colder
Comments
This strain is a tissue culture-adapted version of strain TW-3 (VR-578) which is grown in embryonated chicken eggs.
Before infecting host cells, add 1-mm glass beads and vortex stock material for 45 seconds. Aspirate fluid. Infect shell vial monolayer with 0.2 ml of material.
Centrifuge at 800 x G for 1 hour. Feed with 1.0 ml EMEM (HEPES buffered), 10% FBS (screened for chlamydia antibodies).
Serotype C
Effect on Host
Death of chick embryo
In TC, requires FA to visualize intracellular inclusion bodies, Death in CE
Inclusions detectable by immunofluorescence
Recommended Host
HEp-2 cells (ATCC CCL-23)
chicken embryo, yolk sac; McCoy cells (ATCC CRL-1696); human and nonhuman primate cells
Growth Conditions
Incubation: 2 days at 35-36°C in HEp-2 cells (ATCC CCL-23)
Cryopreservation
Storage temperature: -70°C or lower
Effect on Host
Death of chick embryo
In TC, requires FA to visualize intracellular inclusion bodies, Death in CE
Inclusions detectable by immunofluorescence
Name of Depositor Kuo
Special Collection ATCC
Source
specimen from human conjunctiva, Taiwan
References

Grayston JT, et al. Trachoma-studies of etiology, laboratory diagnosis, and prevention. JAMA 172: 1577-1586, 1960. PubMed: 13829148

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS