Chlamydia suis Everett et al. (ATCC® VR-1474™)

Classification: Chlamydiaceae  /  Product Format: frozen

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification Chlamydiaceae
Agent Chlamydia suis Everett et al.
Strain S45(porcine isolate)
Biosafety Level 2
Isolation
swine intestine, feces and diarrhea fluid
Product Format frozen
Storage Conditions -70°C or colder
Effect on Host
Cytopathic effects (cytocidal and cytoplasmic inclusions) in tissue culture
Death of CE in 4 to 10 days, depending on concentration of inoculum; cytocidal and cytoplasmic inclusions in tissue culture preparation.
Death of chicken embryo in 4 to 10 days, depending on concentration of inoculum
Elementary bodies in Gimenez-stained impression smears from infected yolk sacs of chicken embryos
Tumorigenicity: iodine-positive cytoplasmic inclusions in infected L or BGMk cells;
Recommended Host
HEp-2 cells (ATCC CCL-23)
chicken embryo, yolk sac; buffalo green monkey kidney cells; HEp-2 cells (ATCC CCL-23)
Growth Conditions
Incubation: 4-10 days at 36°C to 37°C in CE; 3 days at 36°C to 37°C in HEp-2 cells (ATCC CCL-23)
Effect on Host
Cytopathic effects (cytocidal and cytoplasmic inclusions) in tissue culture
Death of CE in 4 to 10 days, depending on concentration of inoculum; cytocidal and cytoplasmic inclusions in tissue culture preparation.
Death of chicken embryo in 4 to 10 days, depending on concentration of inoculum
Elementary bodies in Gimenez-stained impression smears from infected yolk sacs of chicken embryos
Tumorigenicity: iodine-positive cytoplasmic inclusions in infected L or BGMk cells;
Name of Depositor J Storz
Special Collection NCRR Contract
Source
swine intestine, feces and diarrhea fluid
References

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

Kaltenboeck B, et al. Structures of and allelic diversity and relationships among the major outer membrane protein (ompA) genes of the four chlamydial species. J. Bacteriol. 175: 487-502, 1993. PubMed: 8419295

type strain

Cross References

Nucleotide (GenBank)
AJ012176
Chlamydia suis partial rnpB gene for RNase P RNA subunit.


Nucleotide (GenBank)
AF269274
Chlamydia suis S45 major outer membrane protein (ompA) gene, partial cds; and tRNA-Gly gene, partial sequence.
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS