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Simkania negevensis Everett et al. (ATCC^® VR-1471^™)

Classification: Simkaniaceae / Product Format: frozen

Simkania negevensis Everett et al. ATCC^® VR-1471^™

frozen

For-Profit: $516.00 Non-Profit: $430.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification	Simkaniaceae
Agent	Simkania negevensis Everett et al.
Strain	Z
Applications	Host of choice: Vero cells (ATCC CCL-81)
Biosafety Level	2
Isolation	contaminated Vero cells, passaged in Israel
Product Format	frozen
Storage Conditions	-70°C or colder

Comments	Antigenic cross-reactivity by immunoblot with chlamydial heatshock proteins
Effect on Host	Cytopathic effects (black granulated cytoplasm, "tar spots", developing to numerous vacuoles, with development of "dancing" particles) Black granulated cytoplasm ("tar spots"), developing to numerous vacuoles, with development of "dancing" particles.

Recommended Host	cells of human and primate origin Vero (ATCC CCL-81)
Growth Conditions	Incubation: 5 days at 35°C to 37°C in Vero cells (ATCC CCL-81)

Effect on Host	Cytopathic effects (black granulated cytoplasm, "tar spots", developing to numerous vacuoles, with development of "dancing" particles) Black granulated cytoplasm ("tar spots"), developing to numerous vacuoles, with development of "dancing" particles.

Name of Depositor	M Friedman
Special Collection	NCRR Contract
Source	contaminated Vero cells, passaged in Israel
References	Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462 Kahane S, et al. Description and partial characterization of a new Chlamydia-like microorganism. FEMS Microbiol. Lett. 109: 329-333, 1993. PubMed: 8339923 type strain
Cross References	Nucleotide (GenBank) AJ012178 Simkania negevensis partial rnpB gene for RNase P RNA subunit.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS