Rickettsia japonica Uchida et al. (ATCC^® VR-1363^™)

Classification: Rickettsiae / Product Format: frozen

Rickettsia japonica Uchida et al. ATCC^® VR-1363^™

frozen

For-Profit: $1,020.00 Non-Profit: $850.00

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Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification	Rickettsiae
Agent	Rickettsia japonica Uchida et al.
Strain	YH
Applications	Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae.
Biosafety Level	3
Isolation	blood of patient with oriental spotted fever, Japan
Product Format	frozen
Storage Conditions	Liquid nitrogen freezer

Comments	Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. Vero cells demonstrated to be mycoplasma-free before infection. Infected Vero cells grow like uninfected cells in MEM with 10% FBS devoid of antibiotics. Subculture by dispersion with 0.05% trypsin and 0.02% EDTA is possible for many passages. Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
Effect on Host	Cytopathic effects in BHK and L929 No observable CPE in Vero Death of CE Fever and scrotal swelling of guinea pig

Comments

Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. Vero cells demonstrated to be mycoplasma-free before infection.

Infected Vero cells grow like uninfected cells in MEM with 10% FBS devoid of antibiotics. Subculture by dispersion with 0.05% trypsin and 0.02% EDTA is possible for many passages.

Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.

Effect on Host

Cytopathic effects in BHK and L929

No observable CPE in Vero

Death of CE

Fever and scrotal swelling of guinea pig

Recommended Host	Vero cells (ATCC CCL-81) BHK-21(C-13) cells (ATCC CCL-10); L-929 cells (ATCC CCL-1); chicken embryo; guinea pig
Growth Conditions	Temperature: 34°C Incubation: 3-6 days

Effect on Host	Cytopathic effects in BHK and L929 No observable CPE in Vero Death of CE Fever and scrotal swelling of guinea pig

Name of Depositor	T Uchida
Source	blood of patient with oriental spotted fever, Japan
References	Uchida T, et al. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42: 303-305, 1992. PubMed: 1581190 Uchida T, et al. Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol. Immunol. 30: 1323-1326, 1986. PubMed: 3553872 type strain

Name of Depositor

T Uchida

Source

blood of patient with oriental spotted fever, Japan

References

Uchida T, et al. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42: 303-305, 1992. PubMed: 1581190

Uchida T, et al. Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol. Immunol. 30: 1323-1326, 1986. PubMed: 3553872

type strain

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Rickettsia japonica Uchida et al. (ATCC® VR-1363™)

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Rickettsia japonica Uchida et al. (ATCC^® VR-1363^™)