Chlamydophila pneumoniae (ATCC® VR-1355)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen

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Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae Grayston et al.
Agent Chlamydophila pneumoniae
Strain TWAR strain 2043
Applications
Respiratory research
Biosafety Level 2
Isolation
nasopharynx of human patient with pneumonia
Product Format frozen
Storage Conditions -70°C or colder
Comments
Forms intracellular inclusions which react with specific anti-C. pneumoniae monoclonal antibodies and monoclonal antibodies directed against the chlamydia LPS (genus antigen: Kallestad C. trachomatis Culture Confirmation System).
Will not stain with iodine or monoclonal antibodies against the MOMP of C. trachomatis. Infection is enhanced by centrifugation and use of cycloheximide (1-2ug/ml) in medium.
This preparation has tested positive for Mycoplasma contamination.
Infection is enhanced by centrifugation and use of cycloheximide (1-2ug/ml) in medium.
Effect on Host
Cytopathic effects (intracellular inclusions) in tissue culture which may be visualized by appropriate conjugated antibodies
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Recommended Host
HEp-2 cells (ATCC CCL-23)
HeLa 229 cells (ATCC CCL-2.1)
human; human cell lines
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 35°C
Incubation: 3 days
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90% - 100% confluent.
Effect on Host
Cytopathic effects (intracellular inclusions) in tissue culture which may be visualized by appropriate conjugated antibodies
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Name of Depositor MR Hammerschlag
Source
nasopharynx of human patient with pneumonia
References

Chirgwin K, et al. In vitro susceptibilities of Chlamydia pneumoniae (Chlamydia sp. strain TWAR). Antimicrob. Agents Chemother. 33: 1634-1635, 1989. PubMed: 2817862

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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