Chlamydophila psittaci (Lillie) Everett et al. ATCC ® VR-125&trade

Permits	These permits may be required for shipping this product: This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Chlamydiaceae
Agent	Chlamydophila psittaci (Lillie) Everett et al.
Strain	Psittacosis strain 6 BC
Biosafety Level	3
Isolation	Parakeet, California, 1941
Product Format	frozen
Storage Conditions	-70° or colder

Comments	Large doses do not affect GP, pigeons or lambs. Feed infected cells with EMEM (HEPES buffered) supplemented with 10% FBS, 5mg/ml glucose, 0.3mg/ml glutamine, 1ug/ml cycloheximide.
Effect on Host	Cytopathic effects (inclusions) in tissue culture Death of chicken embryo

Recommended Host	McCoy cells (ATCC CRL-1696) chicken embryo, yolk sac; mouse, i.c. or i.p. inoculation; parakeet, i.p. inoculation; turkey, i.p. inoculation; chicken embryo in tissue culture; L-929 cells (ATCC CCL-1); McCoy cells (ATCC CRL-1696)
Growth Conditions	Incubation: 4-10 days at 37°C in CE (y.s.); 2 days at 37°C in TC Temperature: 37°C

Effect on Host	Cytopathic effects (inclusions) in tissue culture Death of chicken embryo

Name of Depositor	LA Page
Source	Parakeet, California, 1941
Year of Origin	1941
References	Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. type strain
Cross References	Nucleotide (GenBank) AB001778 Chlamydia psittaci gene for 16S rRNA, strain 6BC. Nucleotide (GenBank) U44032 Chlamydia psittaci putative ribonuclease P RNA gene. Nucleotide (GenBank) X69476 C.psittaci gseA gene for 3-deoxy-D-2-octulosonic acid transferase. Nucleotide (GenBank) AJ012169 Chlamydophila psittaci partial rnpB gene for RNase P RNA subunit. Nucleotide (GenBank) U68447 Chlamydophila psittaci strain 6BC 16S and 23S ribosomal RNA operon, complete sequence.

Permits

These permits may be required for shipping this product:

This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation

Chlamydophila psittaci (Lillie) Everett et al. (ATCC® VR-125™)