Chlamydophila pneumoniae (ATCC® 53592)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae
Agent Chlamydophila pneumoniae
Strain AR-39
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Activities with high potential for aerosol production requires Biosafety Level 3 facilities and practices. Deposited as Chlamydia pneumonia. Prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were referred to as TWAR, after isolates TW-183 and AR-39. TW-183 is available as ATCC® VR-2282™.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Sonicate inoculum for 20 seconds at approximately 240W to disrupt cells. Remove cell growth medium and inoculate with disrupted material. Centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium. Incubate infected culture in a humidified 5% CO2 atmosphere.
Incubation: 3 days
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Name of Depositor Washington Research Foundation
Special Collection NCRR Contract
Source Throat of university student with acute pharyngitis, Seattle, WA, 1983
References

Grayston JT, et al. Chlamydia pnemoniae sp. nov. for Chlamydia sp. Strain TWAR. Int. J. Syst. Bacteriol. 39:88-90, 1989.

Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988.