Escherichia coli (Migula) Castellani and Chalmers (ATCC® 700927)

Strain Designations: EDL 933  /  Type Strain: no  /  Biosafety Level: 2

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Classification Enterobacteriaceae, Escherichia
Strain Designations EDL 933
Application
Enteric Research
Isolation
Derived from existing strain CDC EDL 933, ATCC 43895
Type Strain no
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype O157:H7:K- RefPerna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266, RefPlunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068, RefBurland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640, (Guy Plunkett, personal communication)
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Escherichia coli O157:H7 EDL933, complete genome

Type Strain no
Antigenic Properties Serotype O157:H7:K- RefPerna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266, RefPlunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068, RefBurland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640, (Guy Plunkett, personal communication)
Comments
University of Wisconsin genome project strain
Genome sequenced strain
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor G Plunkett
Special Collection NCRR Contract
Chain of Custody
ATCC <-- G Plunkett <-- C. Kaspar
Isolation
Derived from existing strain CDC EDL 933, ATCC 43895
References

Perna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266

Plunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068

Burland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640

Perna NT, et al. Genome sequence of enterohemorrhagic Escherichia coli O157:H7. Nature 409: 529-533, 2001. PubMed: 11206551

Guy Plunkett, personal communication

Perna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266

Plunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068

Burland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640

Guy Plunkett, personal communication

Perna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266

Plunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068

Burland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640

Guy Plunkett, personal communication

Cross References

Nucleotide (GenBank) : AF071034 LEE element sequence

Nucleotide (GenBank) : AF074613 pO157 plasmid sequence

Nucleotide (GenBank) : AF125520 933W bacteriophage sequence

Nucleotide (GenBank) : AE005174 Escherichia coli O157:H7 EDL933, complete genome

Nucleotide (GenBank) : DQ272420.1 Escherichia coli strain ATCC 700927 clone 2 putative outer membrane lipoprotein (nlpD) gene, partial cds; and truncated sigma 38 factor (rpoS) gene, complete cds

Nucleotide (GenBank) : DQ272419.1 Escherichia coli strain ATCC 700927 clone 1 putative outer membrane lipoprotein (nlpD) gene, partial cds; and truncated sigma 38 factor (rpoS) gene, complete cds

Nucleotide (GenBank) : CP008958.1 Escherichia coli O157:H7 str. EDL933 plasmid, complete sequence

Nucleotide (GenBank) : CP008957.1 Escherichia coli O157:H7 str. EDL933, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation