Rhizobium leguminosarum Jordan (ATCC® 53912)

Strain Designations: pTFX1::10-15 [10-15 DH5alpha, TA1::10-15, TA1::TFX:Tn5, TFX:Tn5; 10-15, pTFX1::Tn5]  /  Type Strain: no  /  Biosafety Level: 1

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Strain Designations pTFX1::10-15 [10-15 DH5alpha, TA1::10-15, TA1::TFX:Tn5, TFX:Tn5; 10-15, pTFX1::Tn5]
Application
Produces protein trifolitoxin, synthesis and resistance genes
Isolation
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Type Strain no
Comments
Recombinant strain expressing trifolitoxin that is capable of promoting the formation of nitrogen fixation nodules.
Production of, and resistance to, trifolitoxin
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Medium ATCC® Medium 111: Rhizobium X medium
Growth Conditions
Temperature: 26.0°C
Name of Depositor Wisconsin Alumni Res. Fndn., DJ Houser, Wisconsin Alumni Res. Fndn.
Isolation
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
References

Triplett EW. Recombinant Rhizobium bacteria inoculants. US Patent 5,183,759 dated Feb 2 1993

Triplett EW. Isolation of genes involved in nodulation competitiveness from Rhizobium leguminosarum bv. trifolii T24. Proc. Natl. Acad. Sci. USA 85: 3810-3814, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation