Rhizobium leguminosarum Jordan (ATCC® 53912)

Strain Designations: pTFX1::10-15 [10-15 DH5alpha, TA1::10-15, TA1::TFX:Tn5, TFX:Tn5; 10-15, pTFX1::Tn5]  /  Type Strain: no  /  Biosafety Level: 1

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Strain Designations pTFX1::10-15 [10-15 DH5alpha, TA1::10-15, TA1::TFX:Tn5, TFX:Tn5; 10-15, pTFX1::Tn5]
Application
Produces protein trifolitoxin, synthesis and resistance genes
Isolation
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Type Strain no
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Type Strain no
Comments
Recombinant strain expressing trifolitoxin that is capable of promoting the formation of nitrogen fixation nodules.
Production of, and resistance to, trifolitoxin
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Medium ATCC® Medium 111: Rhizobium X medium
Growth Conditions
Temperature: 26.0°C
Name of Depositor Wisconsin Alumni Res. Fndn., DJ Houser, Wisconsin Alumni Res. Fndn.
Isolation
Important: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
U.S. Patent Number
References

Triplett EW. Recombinant Rhizobium bacteria inoculants. US Patent 5,183,759 dated Feb 2 1993

Triplett EW. Isolation of genes involved in nodulation competitiveness from Rhizobium leguminosarum bv. trifolii T24. Proc. Natl. Acad. Sci. USA 85: 3810-3814, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation