Deinococcus radiophilus (Lewis) Brooks and Murray (ATCC® 27603)

Strain Designations: RBD [ATCC 27602, ATCC 27604, CCM 2564, NCIB 10648, NCTC 10785, UWO 1055]  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Micrococcus radiophilus Lewis
Strain Designations RBD [ATCC 27602, ATCC 27604, CCM 2564, NCIB 10648, NCTC 10785, UWO 1055]
Application
Produces restriction endonuclease DraI
Produces restriction endonuclease DraII
Produces restriction endonuclease DraIII
Isolation
Irradiated Bombay duck, Harpodon nehereus
Type Strain yes
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Medium ATCC® Medium 679: TGYM medium
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor NF Lewis
Isolation
Irradiated Bombay duck, Harpodon nehereus
References

Curr. Sci. 42: 504, 1973.

Lewis NF. Studies on a radio-resistant coccus isolated from Bombay duck (Harpodon nehereus). J. Gen. Microbiol. 66: 29-35, 1971. PubMed: 4934504

Brooks BW, Murray RG. Nomenclature for "Micrococcus radiodurans" and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int. J. Syst. Bacteriol. 31: 353-360, 1981.

. . FEBS Lett. 180: 219-223, 1985.

Lewis NF, Kumta US. Evidence for extreme UV resistance of Micrococcus sp. NCTC 10785. Biochem. Biophys. Res. Commun. 47: 1100-1105, 1972. PubMed: 5029860

Purvis IJ, Moseley BE. Isolation and characterisation of DraI, a type II restriction endonuclease recognising a sequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA. Nucleic Acids Res. 11: 5467-5474, 1983. PubMed: 6310500

Grosskopf R, et al. Two new restriction endonucleases DraII and DraIII from Deinococcus radiophilus. Nucleic Acids Res. 13: 1517-1528, 1985. PubMed: 2987827

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Cross References

Nucleotide (GenBank) : X52299 Deinococcus radiophilus 5S ribosomal RNA.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation