Fibrobacter succinogenes subsp. succinogenes (Hungate) Montgomery et al. (ATCC® 19169)

Strain Designations: S85 [VPI 12249; L.A. Burkey S85]  /  Type Strain: yes  /  Biosafety Level: 1

Permits And Restrictions

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Deposited As Bacteroides succinogenes Hungate
Strain Designations S85 [VPI 12249; L.A. Burkey S85]
Application
Produces restriction endonuclease FsuI
Produces xylan endo-1,3-beta-xylosidase xylan hydrolase, xylanase
Produces trypsin-resistant xylanases
Biotechnology
Isolation
Bovine rumen
Type Strain yes
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Comments
Medium must be absolutely reduced. Rehydrate vial in 5 mL broth, and immediately dilute 1:5 to a second broth tube. Strain grows best in a stabbed slant or in broth which contains very little cryoprotective agent.
Cellobiose transport
Medium ATCC® Medium 1943: Fibrobacter medium
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 97% CO2-3% H2
Name of Depositor MP Bryant
Chain of Custody
ATCC <-- MP Bryant <-- VPI 12249 <-- K Pittman <-- MP Bryant & LA Burkey S85
Isolation
Bovine rumen
References

Cato EP, et al. Designation of neotype strains for Bacteroides amylophilus Hamlin and Hungate 1956 and Bacteroides succinogenes Hungate 1950. Int. J. Syst. Bacteriol. 28: 491-495, 1978.

Lee SF, et al. Type II DNA restriction-modification system and an endonuclease from the ruminal bacterium Fibrobacter succinogenes S85. J. Bacteriol. 174: 5275-5283, 1992. PubMed: 1644754

Maas LK, Glass TL. Cellobiose uptake by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes. Can. J. Microbiol. 37: 141-147, 1991. PubMed: 2059920

Malburg LM Jr., et al. Fibrobacter succinogenes S85 has multiple xylanase genes. J. Appl. Bacteriol. 75: 564-573, 1993.

Montgomery L, et al. Transfer of Bacteroides succinogenes (Hungate) to Fibrobacter gen. nov. as Fibrobacter succinogenes comb. nov. and description of Fibrobacter intestinalis sp. nov.. Int. J. Syst. Bacteriol. 38: 430-435, 1988.

Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.

Cross References

Nucleotide (GenBank) : M62696 Fibrobacter succinogenes (strain S85) 16S ribosomal RNA.

Nucleotide (GenBank) : M29047 F. succinogenes endoglucanase 3 (cel3) gene, complete cds.

Nucleotide (GenBank) : U05897 Fibrobacter succinogenes S85 endoglucanase E (celE) and endoglucanase D (celD) gene, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation