Vaccinia virus ts mutant ATCC ® VR-3121™

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Poxviridae, Orthopoxvirus
Agent	Vaccinia virus ts mutant
Strain	IHD-W Dts8 (Dales isolate 991)
Applications	The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category L indicating that it produces immature particles with nucleoids and defective membranes with spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5.
Positive for Mycoplasma	No
Biosafety Level	2
Isolation	derived from existing strain
Product Format	frozen
Storage Conditions	-70°C or colder

Comments	Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category L indicating that it produces immature particles with nucleoids and defective membranes with spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5.
Effect on Host	Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing

Recommended Host	Recommended Host: BSC-40 (ATCC CRL-2761
Growth Conditions	Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].

Effect on Host	Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing

Name of Depositor	S Dales, RC Condit, S Dales
Special Collection	NCRR Contract
Source	derived from existing strain
References	This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. cell enlargement, plaques with rounding and eventual sloughing of cells Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Permits

These permits may be required for shipping this product:

Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation

Vaccinia virus ts mutant (ATCC® VR-3121™)