WEHI-3 (ATCC® TIB-68)

Organism: Mus musculus, mouse  /  Tissue: peripheral blood  /  Disease: leukemia

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Organism Mus musculus, mouse
Tissue peripheral blood
Product Format frozen
Culture Properties suspension (some adherent cells)
Biosafety Level 1
Disease leukemia
Strain BALB/c
Applications
Tested and found negative for ectromelia virus (mousepox).
The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Storage Conditions liquid nitrogen vapor phase
Receptor Expression
complement (C3)
Genes Expressed
lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
Comments
The growth of WEHI-3 is inhibited by 4 ng/mL LPS and blocked by higher concentrations.
Dextran sulfate at 30 to 40 µg/mL also inhibits growth.
Latex beads are phagocytized but are not toxic.
Zymosan and BCG are phagocytized and block growth.
The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL.
Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL. Adherent cells may be harvested by scraping.

Medium Renewal: Every 2 to 3 days

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor M Cohn
References

Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787