EL4.BU.1.OUAr.1.1 (ATCC® TIB-41™) Organism: Mus musculus, mouse / Cell Type: T lymphocyte / Disease: lymphoma General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Mus musculus, mouse Cell Type T lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease lymphoma Strain C57BL/6N Storage Conditions liquid nitrogen vapor phase Derivation This line was derived from the EL4 cell line (see ATCC TIB-39). Antigen Expression H-2b; Thy-1.2 Comments This line was derived from the EL4 cell line (see ATCC TIB-39).The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10% Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-3 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Medium Renewal: Every 2 to 3 days Culture Conditions Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% Name of Depositor R Hyman References Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.